diff --git a/Marlin/src/pins/hc32f4/pins_AQUILA_101.h b/Marlin/src/pins/hc32f4/pins_AQUILA_101.h
index 8c3ce10c6d..0b21d9ea19 100644
--- a/Marlin/src/pins/hc32f4/pins_AQUILA_101.h
+++ b/Marlin/src/pins/hc32f4/pins_AQUILA_101.h
@@ -220,10 +220,6 @@
 #define BOARD_USART2_TX_PIN                 PA9
 #define BOARD_USART2_RX_PIN                 PA15
 
-// Unused / Debug
-#define BOARD_USART3_TX_PIN                 PE5
-#define BOARD_USART3_RX_PIN                 PE4
-
 // Onboard LED (HIGH = off, LOW = on)
 #ifndef LED_BUILTIN
   #define LED_BUILTIN                       PA3